Methods: A 70% methanol extract of Spondias pinnata stem bark was studied in vitro for total antioxidant activity, for scavenging of hydroxyl. BACKGROUND: Many diseases are associated with oxidative stress caused by free radicals. Current research is directed towards finding naturally-occurring. BMC Complementary and Alternative Medicine BioMed Central Research article Open Access Antioxidant and free radical scavenging activity of Spondias.

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Finally, sponfias reaction for 20 min and the absorbance was measured against an mixture was diluted to 1 ml with water and the absorb- appropriate blank. The scavenging activity of sample was compared with that of lipoic acid, used as a reference compound.

Reactive oxygen species in living systems: The absorbance of the ABTS. Request Full-text from Authors. Superoxide and singlet oxygen in milk lipid peroxidation. Robak and Glyglewski [ 34 ] reported that flavonoids are effective antioxidants mainly because they scavenge superoxide anions.

The total antioxidant activity of the extract was calculated from the decolorization of ABTS. The aim of the present study was to evaluate the in vitro antioxidant activities of Spondias pinnata stem bark extract.

After incubation for 5 min pinnaha ambient temperature, the absorbance at nm was measured against an appropriate blank to determine the quantity of formazan generated. The production of singlet oxygen 1 O 2 was determined by monitoring NN -dimethylnitrosoaniline RNO bleaching, using a previously reported spectrophotometric method [ 1718 ].

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As shown in figure 7, the Figure 10power assay Reducing reducing power of the plant extract was compared with Reducing power assay. The assay is based on quantification of the degradation product of 2-deoxyribose by condensation with TBA.

Interaction with the extract or standard trolox suppressed the absorbance of the ABTS. Free radicals in biology and medicine.

However, the activities of antioxidants have been attributed to various mechanisms such as prevention of chain initiation, decomposition of peroxides, reducing capacity and radical scavenging [ 44 ].

Superoxide anion is also very harmful to cellular components [ 33 ]. Finally, the reaction mixture was diluted to 1 ml with water and the absorbance was measured at nm. Chap- oxide synthase gene expression. Effects of Spondias pinnata plant Singlet oxygen scavenging assay.

Antioxidant and free radical scavenging activity of Spondias pinnata

Current research is directed towards finding naturally-occurring antioxidants of plant origin. The percentage scavenging of ONOO – was calculated by comparing the results of the test and blank samples.

Robak J, Gryglewski IR: Korycka-Dahl M, Richardson T: S-allylmercaptocysteine scavenges hydroxyl rad- Technol Interaction with the actjvity method of Oyaizu [22] with a slight modification. Pinntaa Hazra Bose Institute India. The data represent the per- lipoic acid. Allergy and Immunology Scavenging effect of Spondias pinnata plant extract and the standard quercetin on superoxide radical. Superoxide and singlet of Similipal biosphere reserve, Orissa, India.

Kasetsart J Nat Sci Antioxidant actions of ovothiol-derived 4-mercaptoimidazoles: Antioxidant and free radical scavenging activity of Spondias pinnata.

Antioxidant and free radical scavenging activity of Spondias pinnata.

Results EDTA was used as a positive control. The data represent the percentage inhibition of deoxyribose degradation. According to the present results, the plant extract inhibits Evans Blue bleaching by scavenging peroxynitrite and its activity is greater than that of the reference gallic acid. Guha Sridhar Pharmaceutical biology Hydrogen peroxide scavenging Hydrogen peroxide scavenging was assayed by the FOX reagent method [ 14 ].

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Antioxidant and free radical scavenging activity of Spondias pinnata.

Manganese dioxide was buffer 20 mM, pH 7. The involvement of low-molecular antioxidants in cross-adaptation of medicine plants to successive action of UV-B radiation and salinity Nataliya RadyukinaV. From the results, it appeared that the H 2 O 2 scavenging activity of the plant extract is negligible compared to that of the standard sodium pyruvate.

All tests were performed six times. The determination of flavo- Rhitajit Sarkar, Dr. Total phenolic content was determined using Folin-Ciocalteu FC reagent according to the method of Singleton and Rossi [ 23 ] with a slight modification.

All tests were performed six Peroxynitrite scavenging times. The nitric oxide generated from sodium nitroprus- many scaavenging antioxidants have been isolated from differ- side reacts with oxygen to form nitrite. The ferrous ion chelating activity was evaluated by a standard method [ 21 ] with minor changes. Yazdanparast R, Ardestani A.