It is thus appropriate to modify as follows the existing Requirements for. Rabies Vaccine for Human Use. General considerations (page 58). Replace the. Three rabies vaccines for human use derived from different rabies virus strains Animals; Biological Assay/methods*; Biological Assay/standards; Biological. BIOLOGICAL ASSAY OF RABIES ANTISERUM: The potency of rabies antiserum is determined by comparing a lethal intracerebral dose of.
|Published (Last):||27 April 2013|
|PDF File Size:||19.7 Mb|
|ePub File Size:||16.84 Mb|
|Price:||Free* [*Free Regsitration Required]|
The toxin should be selected for assay. Prepare mixtures such that 2. Rabies can be transmitted transplacentally Transplants in human- possible Human-to-human: Tetanus anti-toxin is a preparation containing antitoxic globulins that have the power of specifically neutralizing bioaesay toxin formed by clostridium tetani.
Cats are more likely to be aggressive than rabiez Animals exhibit change in instinctive behavior: For this purpose, a suitable preparation of diphtheria toxin is required to be used as a test toxin. Bite from an infected animal. P otency of rabies vaccine is determined by comparing a lethal intra-cerebral dose of a rabies virus with the dose of the standard preparation of rabies vaccines necessary to give for same protection. Suspend the cells in 10ml of sterile suspension medium 1ml of the suspension of cells 10ml sterile suspension mix the inoculums.
Then they are incubated or about 24 hours at about and the diameter or areas of the circular inhibition zones are measured. Mice, 10gg-animal same sex. Great information, I would like to say your post is very informative.
Ratio between two dose of test and two dose of standard should rwbies equal This ratio kept constant through out the assay. The results of this study indicate that the NIH protection test should continue to form the primary basis for potency assay of rabies vaccine as glycoprotein content does not appear to correlate with immunogenic potency for different types of vaccine.
Share to Twitter Share to Facebook.
Prepare fold dilution of std. Consisting free dried synthetic oxytocin peptide with human albumin citric acid Slide Potency is determined by international reference. The potency of rabies antiserum is determined by comparing a or intracerebral dose of rabies virus with the dose of standard preparation of rabies antiserum necessary to biioassay same protection.
Potency of rabies vaccine is determined by comparing the dose necessary to protect mice against a lethal intracerebral dose of rabies vaccine necessary to provide the same protection.
The prepared plates or dishes must be stored such that no significant growth of the test microorganism occurs before use, and the surface of the agar layer is dry at the time of use. The no of units per ml Either it is from horse of other mammals Recommended dose preservatives Slide 9: Virus lifestyle Virus replicated in muscle cells near site of bite for most of incubation time.
Rabies Vaccine Assay Test Animals: Go to Application Have a question? Standard preparation containing freeze dried hyper immune horse serum.
Standard preparation is dried serum or Other preparationthe potency of which has been determined in relation to international standard. You do not have the permission to view this presentation. Oxytocin stimulate the contraction of the bioassaj smooth muscle and the memory gland. The test toxin is allowed to stand for some months before being used for the assay of samples of antitoxin.
Longitudinal Section of Rabies Virus: P return to normal Dilute the preparation with saline solution so as to get same response as standard The ratio between standard and test should be equal If the animal rapidly becomes insensitive to the repeated injection the solution another must be used. HCl [to PH 6. Automatically changes to Flash or non-Flash embed. The presentation is successfully added In Your Favorites.
The Standard Preparation is the Ist International Standard for Diphtheria antitoxin, bioasay, established inconsisting of the dried hyperimmune horse serum and glycerin, or another suitable preparation the potency of which has been determined in relation to the International Standard.
There was a problem providing the content you requested
Centrifuge decant of supernatant liquid [repeat 3 times]. Centrifuge decant supernatant liquid. Solution of known concentration of the std. Go to Application Have a question? Three rabies vaccines for human use derived from different rabies virus strains and prepared on different cell culture substrates were compared with the International Standard for Rabies Vaccine using in vivo and in vitro assay methods in a collaborative study involving 14 participants.
P response Dose should cause decrease in B. The vaccine is prepared immediately before use by reconstitution from the dried vaccine with a suitable sterile liquid. White mice weight 11gg. Presentation Description No description available. These two groups are used for titration of LD50 of challenge suspension.
Rabies VACCINE – JSK NAGARAJAN |authorSTREAM
Dilute the test toxin with saline solution so that 1. Suspend the cells in 10ml of sterile suspension medium 1ml of the suspension of cells 10ml sterile suspension mix the inoculums Slide Acid digested casein solution. I am very happy to your post about on. WordPress Embed Customize Embed. P response Cannulate the crural or brachial vein. Inject mcg of oestradiol benzoate intramuscularly into female rat before the assay Immediately before the assay confirm by vaginal…….
Rabies vaccine is a suspension of a suitable strain of fixed rabies grown in suitable approved cell culture and inactivated by a suitable method.