Several species of Acanthamoeba, including A. culbertsoni, A. polyphaga, A. castellanii, A. astronyxis, A. hatchetti, A. rhysodes, A. divionensis. In order to define the mode by which amoebae recognize ECM components and process this recognition, we analyzed Acanthamoeba culbertsoni attachment. They have been implicated in human infections and disease, including Acanthamoeba culbertsoni, Acanthamoeba polyphaga, Acanthamoeba castellanii.

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Furthermore, soluble factors present in cocultures of A. Acanthamoeba culbertsoni belongs to a group of free-living amoebae, such as Balamuthia mandrillarisNaegleria fowleriand Sappinia pedatathat can cause disease in humans 46 Table 3 Blastogenic response stimulation index of the splenocytes treated with amoeba lysates in mice infected with A.

Meningoencephalitis due to Acanthamoeba SP. J Gen Microbiol ;59 3: Journal of Clinical Microbiology. The serine peptidase activity, however, was associated only with bands of approximate molecular masses of 55 kDa and 50 kDa, compared with A.

It is considered that cell-mediated immune responses should be involved in murine model infected with A. Through the use of class-specific inhibitors, it was demonstrated that both serine and metallopeptidases were released by these amoebae.


Protein measurement with the Folin phenol reagent. In the present study, it was demonstrated that peptidases that are secreted by A. Ferrante A, Thong YH. J Protozool ;15 4: The disparity in molecular masses may be due to the amoeba variants used, to the medium conditions, or to the zymographic analysis applied and attendant extrapolation of molecular masses.

Exp Parasitol ;30 1: Partial characterization of the proteolytic secretions of Acanthamoeba polyphaga. Antimicrobial Agents and Chemotherapy. Each aliquot of medium was analyzed at a protein concentration of 0.

Pathogen & Environment

The lowercase letters and arrows indicate protein species of approximately80, 55, 50, 45, and 30 kDa. Natural killer NK cell activity in the rat.

D Densitometric comparison of lane A1 for A. In vitro destruction of nerve cell cultures by Acanthamoeba spp.: The British Journal of Ophthalmology. Bands indicative of peptidase activity were identified at positions corresponding to molecular masses of approximately,80, 55, 50, 45, and 30 kDa Fig.

Eukaryotic cell encystation and cancer cell dormancy: Gel zymography also was performed on culture supernatants from cocultures of A.

Alternatively, exposure of BV-2 cells to Acanthamoeba spp. This induction of apoptosis on the part of A. Table 5 Blastogenic response stimulation index of the splenocytes treated with con.


Rapid colorimetric assay for cellular growth and survival: Chemokines and cytokines are designated as follows: Activation of the alternative culberttsoni of complement by Acanthamoeba culbertsoni. Blastogenic response of Toxoplasma-infected mouse spleen cells to T- and B-cell mitogens.

Acanthamoeba – Wikipedia

Echinococcus multilocularis metacestode metabolites contain a cysteine protease that digests eotaxin, a CC pro-inflammatory chemokine. In the present study, it is demonstrated that serine peptidases secreted by A.

Immunization with culberrtsoni supernatant in experimental amoebic meningoencephalitis. Phase contrast micrograph of an Acanthamoeba polyphaga cyst. Proteolytic enzymes of pathogenic and non-pathogenic strains of Acanthamoeba spp. Recycling and target binding capacity of human natural killer cells.

Acanthamoeba culbertsoni Elicits Soluble Factors That Exert Anti-Microglial Cell Activity

Trypanosoma cruzi-induced suppressor substance. BV-2 cells express protease-activated receptors PARs 3.

Are the amoebae being credited to an ‘inside job’ done by the host immune response?